Plasmid Buffer Calculator
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THE PIPETTES SOLUTION
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🧬 Plasmid Buffer Calculator
Custom Lab Protocol — Adjusted for 2.5M NaOH & Custom P3
pH must be 8.0. Verify before adding RNase A. Store at 4 °C.
Using 2.5 M NaOH stock. Do not pH-adjust. Store at room temp. Warm to 37 °C if SDS precipitates.
Target pH 4.2. Dissolve GuHCl (endothermic) and KAc in water, adjust pH carefully with glacial acetic acid, then bring to volume.
Kit-specific buffer. Verify against your kit manual. Dissolve GuHCl before adding ethanol.
Stock Solution. You MUST add 4 volumes of 100% ethanol to this stock volume before use.
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