Multi-Insert Assembly Calculator

⚡ Multi‑Insert Assembly

T4 / Gibson / HiFi · molar ratio · vector‑centric

🧬 VECTOR (required)

← amount of vector you want in the reaction (typical 20–100 ng)

✂️ INSERTS (molar ratio relative to vector)

:1

📋 pipetting volumes (20 µl reaction scale*)

Component	Amount (ng)	Volume (µl)

*molar ratio calculation based on vector mass. Use these volumes directly in your assembly mix.