Plasmid Buffer Calculator
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THE PIPETTES SOLUTION
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🧬 Plasmid Buffer Calculator
P1 · P2 · P3 · PB · PE — alkaline lysis + column cleanup
pH must be 8.0. Verify before adding RNase A. Store at 4 °C.
Do not pH-adjust. Store at room temp. Warm to 37 °C if SDS precipitates.
Target pH 5.5. Dissolve potassium acetate in ~80 % water, add acetic acid dropwise, bring to volume.
Kit-specific buffer. Verify against your kit manual.
Kit-specific buffer. Verify against your kit manual.
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