Restriction Digestion Calculator

✂️

NEB Restriction Digest

CutSmart® / NEBuffer  ·  Total = 10 × Buffer 10× Buffer = 2.5 µL

Number of reactions (samples + extra)

reactions

Reaction volume & 10× Buffer

Total (µL) µL 10× Buffer µL

⤳ Total = 10 × Buffer (1× final)

Component setup (per reaction)

🧬 DNA solution

Concentration ng/µL µg/µL

Mass to digest

µg

📦 Calculated volume: 10.00 µL (auto‑filled based on mass & conc)

10× Buffer µL linked to total

Restriction enzyme -- Select enzyme -- µL

ddH₂O µL auto‑fill

✓ All components sum to total.

Total reactions

—

including overage

Total master mix volume

— µL

enough for all reactions

Component	Per Reaction	Master Mix (× N)

📌 Protocol tip: Keep all components on ice. Mix gently. Incubate at the recommended temperature for the specified time.
💡 Always include a no‑enzyme control.

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