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Lab Calculator Suite

8 tools · click to expand

🧬 PCR Setup Q5 · Phusion · SuperFi · LongAmp · XT ▼

Number of reactions (samples + extra)

reactions

PCR enzyme

Q5 Hot Start Q5‑XT LongAmp Taq Phusion SuperFi ~1 U/µL

Reaction volume (µL)

µL

Template DNA (ng per reaction)

ng

Primer (10 µM) each, per reaction

µL each primer

Total master mix

— µL

for N reactions

Component	Per Rx (µL)	Master Mix (×N)

💡 Keep all reagents on ice. Use nuclease‑free water.

📝 IVT Transcription HiScribe · T7‑AG · MegaScript · SP6 ▼

IVT System

HiScribe (conventional) HiScribe T7‑AG MegaScript SP6 IVT

Template DNA (µg)

µg

Reaction volume (µL)

µL

Number of reactions

reactions

Total master mix

— µL

for N reactions

Component	Per Rx (µL)	Master Mix (×N)

💡 Incubate at 37°C for 2‑4 hours. DNase treat after.

💉 Transfection PEI · Lipo · MessengerMax · TransIT ▼

Reagent

PEI (linear, 1 mg/mL) Lipofectamine 3000 MessengerMax TransIT‑2020

DNA/RNA amount (µg per well)

µg

Reagent ratio (µL reagent per µg nucleic acid)

µL/µg default: 3:1

Number of wells

wells

Reagent volume per well

— µL

Total reagent needed

— µL

Diluent (Opti‑MEM)

— µL

Total per well

— µL

💡 Prepare complexes in serum‑free media. Incubate 10‑20 min at RT before adding to cells.

🧫 RNA Extraction — MagMax magnetic beads ▼

Sample volume (per extraction)

µL

Beads ratio (µL beads per µL sample)

µL/µL

Elution volume (µL)

µL

Number of samples

samples

Beads per sample

— µL

Total beads needed

— µL

Wash buffer (×S)

— µL

Elution total

— µL

💡 Use fresh DTT and Proteinase K. Elute with nuclease‑free water.

🦠 Virus Titer Converter PFU · TCID₅₀ · dilution ▼

Input titer

PFU/mL TCID₅₀/mL PFU/µL TCID₅₀/µL

Convert to

PFU/mL TCID₅₀/mL PFU/µL TCID₅₀/µL

Dilution factor (optional, for downstream use)

×

Converted titer

—

Diluted titer

—

💡 TCID₅₀/mL ≈ 0.7 × PFU/mL (for many viruses, use your own conversion factor).

🩸 HA Titer Calculator hemagglutination ▼

Starting dilution (e.g., 1:2, 1:4, 1:8...)

1 : initial

Last well with agglutination (well number, starting at 1)

well #

Dilution factor per well

× 2‑fold

HA Titer

— HAU/50 µL

Reciprocal titer

—

Dilution at last positive well: 1:X

💡 HA titer = highest dilution showing complete agglutination.

🛡️ VNT Calculator virus neutralization ▼

Virus titer (TCID₅₀/mL)

TCID₅₀/mL

Desired TCID₅₀ per well (e.g., 100)

TCID₅₀

Dilution factor for virus stock

× eg. 10⁻³

Number of wells

wells

Virus per well

— TCID₅₀

Total virus needed

— TCID₅₀

Stock volume per well

— µL

Diluent per well

— µL

💡 Prepare virus in serum‑free media. Incubate with serum (1:1) for 1h at 37°C before adding to cells.

🔬 IFA Calculator immunofluorescence ▼

Primary antibody dilution

1 : primary

Secondary antibody dilution

1 : secondary

Volume per well (µL)

µL

Number of wells

wells

Primary Ab per well

— µL

Secondary Ab per well

— µL

Primary total

— µL

Secondary total

— µL

Diluent: PBS/BSA or blocking buffer

💡 Incubate primary 1h at RT or overnight at 4°C. Wash 3× before secondary.

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