ELISA

ELISA Studio · Precision Dilution Lab

Exact pipetting volumes for serial dilution | ng/µL → well concentration

Direct ELISA | Real dilution calculator: from stock to standard curve

Assay & Dilution Setup

ELISA Type: Direct ELISA Indirect ELISA Sandwich ELISA Competitive ELISA

Coating Antibody/Antigen:

Detection Antibody / Conjugate:

Standard Stock Concentration (ng/µL): e.g., PG9 1.21 mg/mL = 1210 ng/µL

Target first standard conc (ng/µL in well): This is the concentration you want in the first well (e.g., 0.5 ng/µL = 500 ng/mL)

Serial dilution factor (fold):

Volume per well (µL): Total volume to add into ELISA plate well

📌 Serial Dilution Pipetting Plan (from stock to plate)

How to prepare: Prepare Std1 by diluting stock with diluent to reach target conc. For subsequent standards: take X µL from previous standard + diluent to make total volume = 100 µL.

Protocol adapts to ELISA type
Direct: enzyme-labeled primary antibody directly detects antigen.

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