AAV Production Calculator

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AAV Production Calculator · Transfection Designer

AAV Production Calculator

PEI transfection · triple plasmid ratio (1:4:8)

Vector : pHelper : Capsid = 1 : 4 : 8 | DMEM & PEI automatically calculated

Volume of media (mL) Make 1:10 scale-up possible — just change mL

Vector / Capsid Variant	Plasmid No. (µg)
AAV1- zsGreen	1788
Chimera 1	—
Chimera 2	—
Chimera 3	—
Chimera 4	—
Chimera 6	—
AAV2/1	—
AAV2/8	—
No capsid plasmid	—

* Plasmids amounts from excel: editable or stored values. Click to edit.

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(curFilter==="All"||p.category===curFilter) && (curSearch===""||p.title.toLowerCase().includes(curSearch)||p.summary.toLowerCase().includes(curSearch))); document.getElementById("protoGrid").innerHTML=filtered.map((p,i)=>`

${p.category}

${p.title}

${p.summary}

`).join(''); } window.toggleProto=function(i){ let el=document.getElementById("protoStep"+i); el.style.display=el.style.display==="none"?"block":"none"; }; function setupProtoFilters(){ let cats=["All",...new Set(protocols.map(p=>p.category))]; document.getElementById("protoFilters").innerHTML=cats.map(c=>`${c}`).join(''); document.querySelectorAll("[data-cat]").forEach(el=>{ el.onclick=()=>{ curFilter=el.getAttribute("data-cat"); renderProtos(); setupProtoFilters(); }; }); } document.getElementById("protoSearch").oninput=(e)=>{ curSearch=e.target.value.toLowerCase(); renderProtos(); }; function calcLog(){ let l=parseFloat(document.getElementById("logTiter").value); if(isNaN(l)) return; let actual=Math.pow(10,l); let t=parseFloat(document.getElementById("targetLog").value); let html=`10^${l.toFixed(4)} = ${actual.toExponential(4)} PFU/mL`; if(!isNaN(t)){ let target=Math.pow(10,t); let red=actual/target; html+=`

Dilution factor: ${red.toFixed(2)}x`; } document.getElementById("logResult").innerHTML=html; } let chart; function genTable(){ let sl=parseFloat(document.getElementById("stockLog").value); let mv=parseFloat(document.getElementById("maxUL").value); let st=parseFloat(document.getElementById("stepSize").value); if(isNaN(sl)||isNaN(mv)) return; let perUL=Math.pow(10,sl)/1000; let vols=[], amts=[]; for(let v=st; v<=mv; v+=st){ vols.push(v); amts.push(perUL*v); if(vols.length>100) break; } let html=`

Virus/µL: ${perUL.toExponential(4)} PFU

`; for(let i=0;i`; html+=`

Volume (µL)	PFU
${vols[i].toFixed(1)}	${amts[i].toExponential(4)}

`; document.getElementById("volResult").innerHTML=html; if(chart) chart.destroy(); let ctx=document.getElementById('volChart').getContext('2d'); chart=new Chart(ctx,{ type:'line', data:{ labels:vols.map(v=>v.toFixed(1)), datasets:[{ label:'PFU', data:amts, borderColor:'#2c7be5', fill:true }] }, options:{ responsive:true } }); } window.copyVol=function(){ let t=document.getElementById("volResult").innerText; navigator.clipboard.writeText(t); alert("Copied"); }; window.exportCSV=function(){ let sl=parseFloat(document.getElementById("stockLog").value); let mv=parseFloat(document.getElementById("maxUL").value); let st=parseFloat(document.getElementById("stepSize").value); if(isNaN(sl)) return; let perUL=Math.pow(10,sl)/1000; let rows=[["Volume_ul","PFU"]]; for(let v=st; v<=mv; v+=st) rows.push([v, perUL*v]); let csv=rows.map(r=>r.join(",")).join("\n"); let a=document.createElement("a"); a.href=URL.createObjectURL(new Blob([csv])); a.download="virus_data.csv"; a.click(); }; function calcDil(){ let s=Math.pow(10,parseFloat(document.getElementById("stockDil").value)); let d=Math.pow(10,parseFloat(document.getElementById("desiredDil").value)); let fv=parseFloat(document.getElementById("finalVol").value); if(isNaN(s)||isNaN(d)||isNaN(fv)) return; if(d>s){ document.getElementById("dilResult").innerHTML="

Desired > stock

"; return; } let volVirus=(d*fv)/s; let volMedia=fv-volVirus; document.getElementById("dilResult").innerHTML=`Virus: ${volVirus.toFixed(2)} µL + Media: ${volMedia.toFixed(2)} µL = ${fv} µL
Dilution factor: ${(s/d).toFixed(2)}x`; } function calcMOI(){ let moi=parseFloat(document.getElementById("moi").value); let cells=parseFloat(document.getElementById("cells").value); let titer=parseFloat(document.getElementById("titerMoi").value); if(isNaN(moi)||isNaN(cells)||isNaN(titer)) return; let pfu=moi*cells; let vol=(pfu/titer)*1000; document.getElementById("moiResult").innerHTML=`${vol.toFixed(2)} µL of virus stock for ${cells.toExponential(2)} cells (MOI=${moi})`; } document.querySelectorAll(".tab-btn").forEach(btn=>{ btn.onclick=()=>{ document.querySelectorAll(".tab-btn").forEach(b=>b.classList.remove("active")); btn.classList.add("active"); document.querySelectorAll(".tab-content").forEach(t=>t.classList.remove("active-tab")); document.getElementById(btn.dataset.tab==="calc"?"calcTab":"protocolsTab").classList.add("active-tab"); if(btn.dataset.tab==="protocols"){ renderProtos(); setupProtoFilters(); } }; }); window.onload=function(){ calcLog(); genTable(); calcDil(); renderProtos(); setupProtoFilters(); }; ' style="width:100%; height:85vh; border:none; border-radius:32px;">

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AAV Production Calculator

AAV Production Calculator

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