🧬 NEBuilder® HiFi Assembly Calculator
📋 ENTER FRAGMENTS 1
| Name | Length | Conc | Type | |
|---|---|---|---|---|
🧪 20 µl REACTION
| Component | Volume | pmols |
|---|---|---|
| TOTAL | 20 µl | 0.000 pmol |
⏱️ 15 min at 50°C
🧊 Store on ice
Protocol: 2-3 frags: 0.05 pmol each • 4-6 frags: 0.2 pmol total •
Incubate 15 min (60 min for >3 frags)