Ligation calculator

🧬 Ligation Ratio Calculator

Insert DNA Length

Unit bp kb

Vector DNA Length

Unit bp kb

Vector DNA Mass

Unit ng µg pg

📏 1:1 = sticky ends

🔗 3:1 = blunt ends

🧪 5:1 = difficult inserts

📊 Required Insert Mass

Formula: Insert Mass = Ratio × Vector Mass × (Insert Length / Vector Length)

💡 Common ratios: 1:1 (cohesive), 3:1 (blunt), 5:1 (problematic)

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