Infusion Cloning Calculator

🧬 In-Fusion™ Calculator

Vector (bp)

Inserts (1-5) 1 2 3 4 5

⚠️ Small fragments (<50bp) may need 3:1 ratio

Vector: 0 ng

Total DNA: 200 ng

Vector: 0 fmol

🧪 10µl Reaction

Vector 0 ng 0 µl

5X Mix 2 µl fixed

Water 8 µl 0 µl

⏱️ 15 min at 50°C

1. Mix components

2. 50°C 15min

3. Transform

💡 5X mix: 2µl | 50ng/µl stock | 15min for <2kb

Go to Link